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. 2022 Dec 13;13:7728. doi: 10.1038/s41467-022-35509-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a ATAC-seq signals in Col and h3.3ko mature seeds over Arabidopsis chromosomes. Signals were calculated in 100 kb bins. Pericentromeric heterochromatin regions are indicated with gray shading. b Metaplot of ATAC-seq signals in Col and h3.3ko mature seeds over all genes. c Heatmap of ATAC-seq signals in Col and h3.3ko mature seeds over all genes. d Genomic distribution of accessible regions in Col and h3.3ko mature seeds. e Genome browser view of ATAC-seq and HTR5-GFP ChIP-seq signals in Col and h3.3ko mature seeds over a few representative loci. AT1G72330: a gene mainly lost accessibility at the 5′ end/promoter region in h3.3ko, AT3G44110: a gene mainly gained accessibility around the 3′ end/genic region in h3.3ko, AT5G03730: a gene with both accessibility increased and decreased regions in h3.3ko. f, g Metaplot of HTR5-GFP ChIP-seq signals in mature seeds over accessibility significantly decreased (f) and increased (g) regions in mature h3.3ko seeds compared with Col. HTR5-GFP ChIP-seq signals over randomly selected regions are served as control.