Table 2.
Summary of the optimisation experiment results
| Volume (µl) | Dilution factor | RCY (%) | CV (%) |
|---|---|---|---|
| 100 | 1/200 | 41.5 | 45.8 |
| 300 | 1/200 | 33.3 | 27.0 |
| 100 | 1/300 | 38.6 | 36.3 |
| 200 | 1/300 | 34 | 20.6 |
| 100 | 1/600 | 37.9 | 52.8 |
| 300 | 1/600 | 38.5 | 33.8 |
| 150 | 1/500 | 41.0 | 18.4 |
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