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. 2022 Dec 13;12:21559. doi: 10.1038/s41598-022-26173-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Combined AFM/CLSM images of Rhodococcus cells after 9 days of n-hexacosane biodegradation. Experimental variants: (a) R. opacus IEGM 262 grown in the presence of 0.2% (w/w) n-hexacosane; (b) R. opacus IEGM 262 grown in the presence of 0.2% (w/w) D-glucose (biotic control); (c) R. erythropolis IEGM 212 grown in the presence of 0.2% (w/w) n-hexacosane; (d) cell-free medium with 0.2% (w/w) n-hexacosane (abiotic control). Cells are stained with LIVE/DEAD® BacLight™ Bacterial Viability Kit: green—living cells, mixed green/red—living but partially damaged or unstained cells, black—abiotic objects (hydrocarbon particles). Arrows show hydrocarbon particles.