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. 2022 Nov 28;19(11):853–866. doi: 10.11909/j.issn.1671-5411.2022.11.002

Figure 7.

Figure 7

Adenosine monophosphate-activated protein kinase plays an important role in the antihypertrophic effect of 7,8-DHF.

(A): Representative Western blots analysis and the quantification of AMPK and its phosphorylation in ISO-induced H9c2 cells. N = 6 in each group. (B): Representative Western blot bands and quantification of p-STAT3/STAT3. N = 6 in each group. (C): Effect of compound C (10 μmol/L, 12 h) on the antihypertrophic role of 7,8-DHF in ISO-induced H9c2 cells. N = 5 in each group. (D): Effect of compound C on the antioxidative role of 7,8-DHF in ISO-induced H9c2 cells. Red fluorescence indicates mitochondrial ROS, whereas blue fluorescence indicates nuclei. Scale bar: 10 μm, n = 6 in each group. Data are expressed as mean ± SE. *P < 0.05, **P < 0.01 vs. control, #P < 0.05, ##P < 0.01 vs. ISO, &P < 0.05, &&P < 0.01 vs. +7,8-DHF. ISO: isoproterenol; 7,8-DHF: 7,8-dihydroxyflavone.