Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description | References or sources |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169(Φ80lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Hanahan (1983) |
| Acidovorax citrulli | ||
| Aac5 | Wild-type watermelon strain; Apr | Yan et al. (2013) |
| ΔbarAAc | barAAc mutant, Apr | This study |
| ΔbarAAccomp | ΔbarAAc complementation strain, ΔbarA containing pBBR-Aave_2063; Apr, Kmr | This study |
| WT-pB | Aac5 containing pBBR1MCS-2; Apr, Kmr | This study |
| ΔbarAAc-pB | ΔbarAAc containing pBBR1MCS-2; Apr, Kmr | This study |
| WT-EV | Aac5 containing pBBRNolac-4FLAG; Apr, Kmr | This study |
| WT-hrpG | Aac5 containing pBBR-4FLAG-hrpG; Apr, Kmr | This study |
| ΔbarAAc-hrpG | ΔbarAAc containing pBBR-4FLAG-hrpG; Apr, Kmr | This study |
| WT-hrpX | Aac5 containing pBBR-4FLAG-hrpX; Apr, Kmr | This study |
| ΔbarAAc-hrpX | ΔbarAAc containing pBBR-4FLAG-hrpX; Apr, Kmr | This study |
| WT-GUS | Aac5 containing pBBRNolacGUS; Apr, Kmr | This study |
| WT-hrpGp-GUS | Aac5 containing pBBR-GUS-hrpGp; Apr, Kmr | This study |
| ΔbarAAc-hrpGp-GUS | ΔbarAAc containing pBBR-GUS-hrpGp; Apr, Kmr | This study |
| WT-hrpXp-GUS | Aac5 containing pBBR-GUS-hrpXp; Apr, Kmr | This study |
| ΔbarAAc-hrpXp-GUS | ΔbarAAc containing pBBR-GUS-hrpXp; Apr, Kmr | This study |
| Plasmids | ||
| pK18mobsacB | Cloning and suicide vector with sacB for mutagenesis; Kmr | Schäfer et al. (1994) |
| pK18-Aave_2063-Up&Down | pK18mobsacB carrying 563-bp upstream and 550-bp downstream sequences of Aave_2063; Kmr | This study |
| pBBR1MCS-2 | Broad-host range expression vector; Kmr | Kovach et al. (1994) |
| pBBR-Aave_2063 | pBBR1MCS-2 carrying 5,534-bp coding region of Aave_2063; Kmr | This study |
| pBBRNolac-4FLAG | lac promoter was deleted from pBBR1MCS-2 and C-terminal 4 × FLAG tag was inserted; need the native promoter to drive expression; Kmr | Zhang et al. (2018) |
| pBBR-4FLAG-hrpG | pBBRNolac-4FLAG carrying 1,336-bp hrpG sequence of A. citrulli; Kmr | This study |
| pBBR-4FLAG-hrpX | pBBRNolac-4FLAG carrying 1,991-bp hrpX sequence of A. citrulli; Kmr | This study |
| pBBRNolacGUS | lac promoter was deleted from pBBR1MCS-2 and GUS reporter gene was inserted; Kmr | Zhang et al. (2018) |
| pBBR-GUS-hrpGp | pBBRNolacGUS carrying 468-bp promoter sequence of hrpG; Kmr | This study |
| pBBR-GUS-hrpXp | pBBRNolacGUS carrying 486-bp promoter sequence of hrpX; Kmr | This study |
| pRK600 | Helper strain used in tri-parental mating; Cmr | Lab collection |
Kmr, Apr, and Cmr indicate resistant to kanamycin, ampicillin, and chloramphenicol, respectively.