Table 2.
Kinetic and thermodynamic parameters for [1–339] and [35–339] wild-type AnxA2 and the indicated mutant AnxA2 variants
| kD-I (s−1) | kN-D (10−5 s−1) | KI-N (104) | ΔΔGD-N (kcal mol−1) | ΔΔGD-I (kcal mol−1) | |
|---|---|---|---|---|---|
| [1–339] wild-type | 6.5 ± 1.0 | 1.3 ± 0.7 | 0.2 ± 0.1 | ||
| [1–339] F33A-D38A-R63A-Q67A | 1.3 ± 0.2 | 0.8 ± 3.7 | 5.5 ± 0.5 | 0.7 ± 0.2 | 2.5 ± 0.3 |
| [1–339] D38A | 1.1 ± 0.2 | 1.2 ± 0.6 | 2.3 ± 0.2 | 1.0 ± 0.2 | 2.4 ± 0.3 |
| [1–339] V57E | 2.8 ± 0.5 | 2.1 ± 1.0 | 6.3 ± 0.6 | 0.8 ± 0.2 | 2.7 ± 0.3 |
| [1–339] R63A | 3.7 ± 0.6 | 1.3 ± 0.6 | 1.9 ± 0.2 | 0.3 ± 0.2 | 1.6 ± 0.2 |
| [1–339] Q67A | 2.2 ± 0.4 | 4.8 ± 3.2 | 0.2 ± 0.1 | 1.4 ± 0.2 | 1.4 ± 0.2 |
| [1–339] V98R | 2.9 ± 0.5 | 60 ± 20 | 0.6 ± 0.1 | 2.7 ± 0.3 | 3.3 ± 0.3 |
| [1–339] G101Y | 3.0 ± 0.5 | 1.7 ± 0.8 | 1.7 ± 0.2 | 0.6 ± 0.1 | 1.8 ± 0.2 |
| [35–339] wild-type | 1.4 ± 0.2 | 2.4 ± 1.0 | 0.9 ± 0.1 | 1.2 ± 0.2 | 2.0 ± 0.2 |
| [35–339] R63A | 1.4 ± 0.2 | 2.9 ± 1.3 | 4.2 ± 0.4 | 1.4 ± 0.2 | 3.0 ± 0.3 |
| [35–339] Q67A | 1.3 ± 0.2 | 3.2 ± 1.4 | 1.8 ± 0.2 | 1.5 ± 0.2 | 2.7 ± 0.3 |