TABLE 3.
Primary sequence and antipseudomonal activities of CAP18 truncated peptidesa
| Peptide | Peptide sequence | EC50 (μM) against P. aeruginosa PAO1
|
|
|---|---|---|---|
| Low salt | High salt | ||
| CAP18 | GLRKRLRKFRNKIKEKLKKIGQKIQGLLPKLAPRTDY | 0.22 ± 0.05 (6) | 0.11 ± 0.02 (4) |
| CAP1822 | RKRLRKFRNKIKEKLKKIGQKI | 0.49 ± 0.17 (4) | 0.31 ± 0.04 (4) |
| CAP1821a | GLRKRLRKFRNKIKEKLKKIG | 0.22 ± 0.03 (3) | 0.21 ± 0.04 (3) |
| CAP1818 | KRLRKFRNKIKEKLKKIG | 0.46 ± 0.16 (3) | 0.70 ± 0.13 (3) |
| CAP1815a | RKFRNKIKEKLKKIG | 0.82 ± 0.23 (3) | >10.6 (3) |
| CAP1819 | LRKFRNKIKEKLKKIGQKI | 0.69 ± 0.11 (3) | 1.28 ± 0.15 (3) |
| CAP1821b | LRKFRNKIKEKLKKIGQKIQG | 0.72 ± 0.20 (4) | 1.35 ± 0.01 (2) |
| CAP1813 | KIKEKLKKIGQKI | 14.8 ± 0.90 (3) | >16.1 (3) |
| CAP1815b | KIKEKLKKIGQKIQG | 5.92 ± 0.17 (3) | >46.0 (3) |
| CAP1817 | KIKEKLKKIGQKIQGLL | 1.19 ± 0.11 (3) | >7.9 (3) |
a
Activities were determined by the luminescence assay under low-salt (25 mM NaCl) or high-salt (175 mM NaCl) conditions. Calculated molecular weights for the peptides are as follows: CAP18, 4,434; CAP1821a, 2,610; CAP1818, 2,284; CAP1815a, 1,886; CAP1822, 2,810; CAP1819, 2,369; CAP1821b, 2,554; CAP1813, 1,554; CAP1815b, 1,739; and CAP1817, 1,966.
b
Data are mean EC50 ± standard error of the mean, and the number of experiments (n) is indicated.