FIGURE 2.

Irigenin promoted cell cycle arrest at G2/M of GBM cells. (A) Flow cytometry assay with PI staining showed the percentage of each cell phase in DBTRG and C6 cells treated with 50 μM IR for 24 h (B–C) Quantification of cell cycle percentage in DBTRG cells (B) and C6 cells (C) as shown in (A) (n = 3, per group). (D) Western blot detected the expression of Cyclin B1 in DBTRG cells treated with 50 μM IR. (E) Quantification of the relative CyclinB1 level as shown in (D) (n = 3, per group, normalized to control). Data were shown as mean ± SEM. ^* p < 0.05, ^*** p < 0.001, compared with control treatment.