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. 2022 Nov 30;13:1027577. doi: 10.3389/fphar.2022.1027577

FIGURE 3.

FIGURE 3

Irigenin induced the apoptosis of GBM cells. (A) Flow cytometry was performed to evaluate the apoptotic ratio in DBTRG and C6 cells treated with 50 μM IR and the control. (B–C) Quantification of the percentage of apoptotic cells in DBTRG (B) and C6 cells (C) (n = 3, per group). (D) Immunostaining analysis of cleaved-Caspase 3 (green) in DBTRG and C6 cells treated with 40 μM IR for 24 h (E–F) Quantitative analysis of the percentage of cleaved-Caspase 3-positive cells over total DBTRG (E) and C6 (F) cells in one field as shown in (D) (n = 13, per group). (G) Propidium iodide (PI) staining of DBTRG cells and C6 cells treated with 50 μM IR for 24 h (H–I) Quantitative analysis of the percentage of PI+ cells over total DBTRG (H) and C6 (I) cells as shown in (G) (n = 16, per group). (J) Western blot detected the expression of Bcl-2, Bax and cleaved-Caspase 3 in DBTRG cells treated with IR. (K–M) Quantification of the relative Bcl-2 (K), Bax (L), and cleaved-Caspase 3 (M) level as shown in (J) (n = 3, per group, normalized to control). Scale bars, 20 μm in figure (D) and 50 μm in figure (G). Data were shown as mean ± SEM. * p < 0.05, *** p < 0.001, compared with control treatment.