FIGURE 5.

Irigenin downregulated YAP signaling in GBM cells. (A) Immunostaining of YAP in DBTRG and C6 cells treated with 40 μM IR. (B–C) Quantification of the intensity of YAP in DBTRG cells (B) and C6 cells (C) as shown in (A) (n = 16, per group). (D) Western blot detected the expression of p-MOB1/MOB1 and p-YAP/YAP in DBTRG cells treated with 50 μM IR. (E–F) Quantification of the relative level of p-MOB1/MOB1 (E) and p-YAP/YAP (F) as shown in (D) (n = 3 per group, normalized to control). Scale bars, 20 μm. Data were shown as mean ± SEM. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, compared with control treatment.