FIGURE 6.

Irigenin downregulated the β-catenin signaling pathway in GBM cells. (A) Immunostaining of β-catenin in DBTRG and C6 cells treated with 40 μM IR. (B–C) Quantification of the intensity of β-catenin in DBTRG cells (B) and C6 cells (C) as shown in (A) (n = 10, per group). (D) Western blot detected the expression of β-catenin and Cyclin D1 in DBTRG cells treated with 50 μM IR. (E–F) Quantification of the relative level of β-catenin (E), and Cyclin D1 (F) as shown in (D), (n = 3 per group, normalized to control). Scale bars, 20 μm. Data were shown as mean ± SEM. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, compared with control treatment.