FIGURE 7.

Overexpression of YAP partially rescued cell viability and proliferation inhibited by irigenin. (A) The cell viability by CCK-8 in C6 cells transfected with GFPN1 or GFP-YAP for 48 h and then treated with 50 μM IR and the control (n = 3, per group). (B) Immunostaining of PH3 (red) in C6 cells was transiently transfected with GFPN1 or GFP-YAP for 48 h and then treated with 40 μM IR. The white arrow indicated transfected with GFPN1 or GFP-YAP and PH3⁺ cells. (C) Quantitative analysis of the percentages of PH3⁺ cells over total C6 cells as shown in (B) (n = 10, per group). (D) Typical images of C6 cells transfected with GFPN1 or GFP-YAP then treated with 50 μM IR in a transwell assay. (E) Quantification of the number of migrated cells as shown in (D) (n = 8, per group). (F) Typical images of C6 cells transfected with GFPN1 or GFP-YAP when treated with 25 μM IR in wound healing assay. (G) Quantification of wound healing percentage as shown in (F) (n = 13, per group). (H) Western blot detected the expression of YAP and β-catenin in C6 cells transfected with GFPN1 or GFP-YAP and then treated with IR. (I–J) Quantification of the relative level of YAP (I) and β-catenin (J) as shown in (H) (n = 4/3 per group). Scale bars, 20 μm in Figure (B), 100 μm in Figure (D) and (F). Data were shown as mean ± SEM. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, compared with control treatment.