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. 2022 Nov 30;13:1050906. doi: 10.3389/fgene.2022.1050906

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Copyright © 2022 Bao, Zhang, Liu, Wu and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The circCDH2-miR-33b-3p-ULK1 interaction axis may be associated with autophagy. (A) The protein expression and phosphorylation (S555) of ULK1 were determined using western blotting. (B) Co-immunofluorescence staining for ULK1 (red) and LC3 (green) in placental sections from control and GDM patients is shown. Nuclei were stained with DAPI. Scale bars=100 μm. (C) The relative expression of ULK1 mRNA in HG-treated HTR-8/SVneo cells and the inhibitory effects of siRNAs on circCDH2 expression were examined were examined by qPCR. (D) Western blot analysis showed the expression of LC3 and ULK1 in cells treated with HG.