FIG. 3.

Effect of cytotoxicity of wtVT1 on viability of Caco-2 and Vero cells. (A) Caco-2 (■) and Vero cells (●) were treated with 100 pM wtVT1 for the indicated periods. The relative cell viability of these cells was calculated. Means ± standard deviations (n = 3) are shown. (B) Caco-2 cells were incubated with 100 pM wtVT1 (■), 100 pM mutVT1 (●), 10 μg of cycloheximide per ml (▴), or 10 μg of anisomycin per ml (○) for the periods indicated. The relative incorporation of [³⁵S]methionine compared to that in PBS-treated Caco-2 cells is shown. Means ± standard deviations (n = 3) are shown.