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. 2022 Dec 13;10(12):e005088. doi: 10.1136/jitc-2022-005088

Figure 1.

Figure 1

SP140 expressed heterogeneously in HNSCCs and its high expression in TAMs is associated with a favorable survival and high tumor mutation burden. (A) SP140 expression in primary HNSCC tumors and normal adjacent tissue in TCGA data (n=520 HNSCC, n=44 normal). (B) SP140 expression versus TP53 mutation status in patient with HNSCC tumors in the TCGA dataset (n=327, TP53 mutant, and n=175, TP53 non-mutant). (C) SP140 expression in primary HPV+ HNSCC tumors, HPV− HNSCC tumors, and normal adjacent tissue in the TCGA data (n=44, normal; n=80, HPV+ HNSC, and n=434, HPV− HNSCC). (D) Representative images of SP140 expression in HNSCC TAMs (CD68+) of normal tissues and HNSCC tumor tissues. (E) Mean fluorescent intensity was calculated in TAMs (CD68+) by quantification of SP140 expression using QuPath software and compared between controls, patients with HNSCC with unfavorable 5-year survival, and patients with HNSCC with favorable 5-year survival. (F) Correlation of SP140 expression and number of non-silent mutations in 497 HNSCCs in the TCGA dataset. (G) Correlation of SP140 expression and number of non-silent mutations in over 9766 cancers in the TCGA dataset. (A–C) Box and whisker plots, with whiskers representing minimum and maximum for the panel. (E) Scatter plot (median and 95% CI). P values (A–C) were calculated by Student’s t-test and corrected for multiple comparison by Bonferroni correction. The correlation between SP140 and mutation burden was quantified with Pearson’s correlation coefficient and corrected for multiple comparison. ***P< 0.001, ****P< 0.0001 after correction for multiple comparisons. The scale bars indicate 100 μm in the left images and 30 μm in the right images. HNSCC, head and neck squamous cell carcinoma; HPV, human papillomavirus; RSEM, RNA-Seq by Expectation-Maximization; SP140, speckled protein 140; TAM, tumor-associated macrophage; TCGA, The Cancer Genome Atlas.