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. 2022 Dec 13;10(12):e005088. doi: 10.1136/jitc-2022-005088

Figure 7.

Figure 7

High expression of SP140 in tumors is associated with a favorable response to immunotherapy. (A) RNA was isolated from pretreatment specimens of HNSCC and lung cancer responders and non-responders to anti-PD-1 immunotherapy. RNA was isolated and levels of SP140 were quantified by RT-qPCR (n=21). 18S was used for normalization. Student’s t-test was used for statistical analysis. (B, C) Receiver operating curves of SP140 and PD-L1 for discriminating responders or non-responder cases. Y-axis represents sensitivity (%) and X-axis represents 100% specificity (%). (D) Patients with metastatic melanoma were dichotomized to high expression and low expression groups based on expression of SP140 (the highest quartile vs the rest). Overall survival of patients with high levels of SP140 (n=12) and low levels of SP140 (n=28) was graphed and analyzed using the Kaplan-Meier estimate. (E, F) Tumors with high expression of SP140 (n=12) versus tumors with low levels of SP140 (n=28) showed higher infiltration of M1 macrophages, CD8 T cells, gamma delta T cells, and overall immune score. The Wilcoxon test was used for statistical analysis, and the p value was corrected for multiple comparisons. **P<0.01, ***P<0.001, ****P<0.0001. HNSCC, head and neck squamous cell carcinoma; M1, proinflammatory phenotype; PD-1, programmed cell death protein 1; PD-L1, programmed death-ligand 1; RT-qPCR, reverse transcription–quantitative PCR; SP140, speckled protein 140.