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. 2022 Nov 30;13:1065253. doi: 10.3389/fpls.2022.1065253

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Copyright © 2022 Li, Liu, Mei, Jiang, Lv, Zhai and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evolutionary analysis of OCP and its orthologous proteins. (A) Structures of OCP. (B) Phylogenetic tree of OCP and its orthologous proteins in five plant species. Based on a shared amino acid sequence identity greater than 50% with OCP, 27 amino acid sequences were collected from NCBI. The neighbor-joining tree was built using the Muscle program, with 1000 bootstrap replicates, in MEGA7. Protein structure was analyzed by SMART. All the proteins contained the same four domains, signal peptide, inhibitor I29, pept_C1, and GRAN. (C) Comparison of the amino acid sequences of OCP with RD21B, RD21A, and CYP1. The black coloring shows the same amino acids present in the four proteins. The * indicates the amino acid position number, 10, 30, 50......