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. 2022 Nov 30;13:1065253. doi: 10.3389/fpls.2022.1065253

Figure 5.

Figure 5

OCP is unable to form a complex with OsRACK1A or OsSNAP32, or affect the stability of either. (A) Subcellular localization of OsRACK1A and OsSNAP32 in rice protoplasts. Scale bar = 10 μm. (B) Subcellular co-localization of OCP and OsRACK1A or OsSNAP32 in rice protoplasts. Scale bar = 10 μm. (C) Yeast two-hybrid assays showing the non-interaction of OsRACK1A and OsSNAP32; pGBKT7-53 and pGADT7-T were used as positive controls, while pGBKT7-Lam and pGADT7-T were used as negative controls. Yeast three-hybrid assays showed that OCP, OsRACK1A, and OsSNAP32 could not form a complex. (D) Relative transcript levels of OsRACK1A and OsSNAP32 in ocp-ko1 and TP309 were assessed by qRT-PCR (mean ± SD, n = 3). This experiment was repeated twice, ** P ≤ 0.01, t test. (E) Detection of the influence of OCP on the stability of OsRACK1A and OsSNAP32. EGFP-His served as the negative control. GST-OsRACK1A and GST-OsSNAP32 were detected with Anti-GST.