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. 2022 Dec 14;24:273. doi: 10.1186/s13075-022-02964-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The miR-187-3p links Satb2 and circ-Iqsec1. A Venn diagram showing that miRNA interacts with both Satb2 and circ-Iqsec1. B RT-qPCR data giving nine miRNA expressions in BMSCs post osteogenic induction for 0 (NC) and 3 (T) weeks. C Bioinformatics analyses predicting miR-187-3p binding sites in circ-Iqsec1. MUT version regarding circ-Iqsec1 is provided. D Relative luciferase activity 2 d post HEK293T cell transfection with miR-187-3p mimic/NC or circ-Iqsec1 WT/MUT. E miR-187-3p binding site predictions regarding Satb2 3'-UTR. Mutant 3′-UTR-Satb2 version is given. F Relative luciferase activity 2 days post-HEK293T cell transfection through miR-187-3p mimic/NC or 3′-UTR-Satb2 WT/Mut. G–I RT-qPCR results giving circ-Iqsec1, miR-187-3p, and Satb2 expressions in BMSCs after transfection with si-circ-Iqsec1, miR-187-3p inhibitor, or Satb2 overexpression vector alone or in combination. Outputs are expressed as the mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circ-Iqsec1