TABLE 1.
Primers used for pGL4.10 reporter plasmids constructions
| Gene | Primer sequence (5′–3′) by directiona |
|
|---|---|---|
| Forward | Reverse | |
| G6pc promoter | CTGGTACCAGCCAAGACTGGCAGATCTCT | CGCTCGAGCATCCTCATTTCCTTGGCACCT |
| PEPCK promoter | GCCTCGAGTTGACAAGGGACAGTTGC | CGCAGATCTTGGATGATCTCGAAGGGA |
| G6pc CRE mutant | ||
| Sequence 1 | CCTCTAGACTCGAGGCCACCATGGAGGAAGGAATGAATGTTC | GTAGGTTGTCCCAAACATGTTCAGGGTGATTTAGCAAAAATAGAAAAACAGGC |
| Sequence 2 | CTATTTTTGCTAAATCACCCTGAACATGTTTGGGACAACCTACTGGTGATGCA | CCGAGCTCGGATCCCAACGACTTCTTGTGCGGCTGG |
| PEPCK CRE mutant | ||
| Sequence 1 | TCGCTAGCCTCGAGTTGACAAGGGACAGTTGC | CGCCACTGTTTTCAGGGGGCAGGTCTCTG |
| Sequence 2 | CCCCCTGAAAACAGTGGCGAGCCTCCCTG | CCGAGGCCAGATCTTGGATGATCTCGAAGGGA |
a
Mutated sites are marked with an underline.