FIG 7.

CHIKV-nsP2 interacts with the host Chk2 and Chk1 proteins. The Vero cells were infected with CHIKV and harvested at 6 hpi. The cell lysates were coimmunoprecipitated with the Chk2 and Chk1 antibodies, respectively. (A) Western blot analysis depicting the levels of nsP2, nsP1, E2, Chk2, and GAPDH in the whole-cell lysate (left) where GAPDH was used as a control, and coimmunoprecipitation analysis showing the interaction of the CHIKV-nsP2 and Chk2 proteins (middle). Right panel represents the negative control, where normal mouse IgG was used to immunoprecipitate the protein complex and was probed with the nsP2 antibody. (B) Western blot analysis depicting the levels of nsP2, nsP1, E2, Chk1, and GAPDH in the whole-cell lysate (left) where GAPDH was used as a control, and coimmunoprecipitation analysis showing the interaction of the CHIKV nsP2 and Chk1 proteins (middle). Right panel represents the negative control where normal mouse IgG was used to immunoprecipitate the protein complex and was probed with the nsP2 antibody.