TABLE 2.
Combinations of primers used for cloning constructs
| Construct | PCR run | Primer name | Template | Source |
|---|---|---|---|---|
| pLV-SIN-TetOne-ZeoR (pSTZ) | 1 | 169, 171 | pLVX-TetOne-Zeo | 58 |
| 2 | 170, 172 | |||
| Overlap | 169, 170 | PCR 1 and 2 | ||
| shREN | N/A | 161, 162 | pZIP-ZsGreen-T2A-Hyg-shRen.713 | 48 |
| BCL2 | N/A | 50, 51 | pCDNA3 HA-BCL2 | 59 |
| BCL-xL | 1 | 54, 57 | pcDNA3 HA-BCL-xL | |
| 2 | 55, 56 | |||
| Overlap | 54, 55 | PCR 1 and 2 | ||
| BCL-W | 1 | 62, 65 | pLX304 BCL2L2 | DNASU |
| 2 | 63, 64 | |||
| Overlap | 62, 63 | PCR 1 and 2 | ||
| vBCL2 N-FLAG-HA | 1 | EHG4087, 67 | pLC-FLAG-HA-vIRF3-P2A-hyg | M. Manzano and E. Gottwein, unpublished |
| 2 | 66, 69 | BC-1 genomic DNA | ||
| 3 | 68, 70 | BC-1 genomic DNA | ||
| 1 + 2 | EHG4087, 69 | PCR 1 and 2 | ||
| Final | EHG4087, 70 | PCR 1 + 2 and 3 | ||
| BAX g2 | Anneal | 97, 98 | ||
| BAX g3 | Anneal | 99, 100 | ||
| BAK1 g1 | Anneal | 5, 6 | ||
| BAK1 g2 | Anneal | 103F, 103R | ||
| BCL2 g1 | Anneal | 197, 198 | ||
| BCL2 g2 | Anneal | 199, 200 | ||
| BCL-W g1 | Anneal | 219, 220 | ||
| BCL-W g2 | Anneal | 221, 222 |