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. 2022 Dec 14;12:21614. doi: 10.1038/s41598-022-25423-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Immunofluorescence images presenting a difference in the multipotency of hNTSCs from different donors. (B) Dot plot presenting expression of stemness-related genes within five different cells: the frequency of cells within a cluster expressing the gene of interest (dot size) and the level of expression of the gene (the degree of color intensity) are visualized. (C) Principal component analysis (PCA) and (D) scree plots for PCA. (E) PCA loading plot presenting the transformation factors of PCA. (F) Functional enrichment analysis visualized by each gene ontology (GO) term in which each node is linked according to a kappa score of ≥ 0.4. FDR.adj.p-value (FDR adjusted p-value). (G) Statistical significance and number of each GO term are presented via functional enrichment analysis. P-values were adjusted with FDR.