Table 1. Primer sequences for a real-time PCR assay.
Gene | Primer pair sequences | Gene ID |
TNF-α | F: 5`-AAATGGGCTCCCTCTCATCAGTTC-3` | X66539 |
R: 5`-TCTGCTTGGTGGTTTGCTACGAC-3` | ||
TNFR1 | F: 5`-CCAAGTGCCACAAAGGAACC-3` | NM_013091.2 |
R: 5`-TCAGGTAGCGCTGGAATTGG-3` | ||
Nrf2 | F: 5`-AGCAGGACATGGATTTGATT-3` | XM_032903520.1 |
R: 5`-CTTCTCCTGTTCCTTCTGGA-3` | ||
HO-1 | F: 5`-ACAGAAGAGGCTAAGACCG-3` | XM_032887931.1 |
R: 5`-CAGGCATCTCCTTCCATT-3` | ||
GAPDH | F: 5′-CCATCAACGACCCCTTCATT-3′ | NM_017008 |
R: 5′-CACGACATACTCAGCACCAGC-3′ |