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. 2022 Nov 23;9:986022. doi: 10.3389/fmolb.2022.986022

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Copyright © 2022 Schifano, Conta, Preziosi, Ferrante, Batignani, Mancini, Tomassini, Sciubba, Scopigno, Uccelletti and Miccheli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lifespan analysis and fat accumulation in pep-2 mutants. (A) Kaplan-Meier survival plot of pep-2 mutant worms supplemented with 10 mM 2-HIBA. Lifespans of untreated worms were reported as controls; n = 60 for each data point of single experiments (ns: not significant). The assay was performed in triplicate. (B) BODIPY staining of 1 day adult pep-2 worms treated or not with 10 mM 2-HIBA. Scale bar = 50 μm. (C) Percentage of Lipid Droplets (LDs) and (D) Mean Fluorescence Intensity (MFI) of fat accumulation observed by BODIPY staining. Statistical analysis was evaluated by one-way ANOVA with the Bonferroni post-test; asterisks indicate significant differences (**p < 0.01, ***p < 0.001). Bars represent the mean of three independent experiments. (E) Expression of sams-1, sbp-1, fat-7, and acs-2 genes in 10 mM 2-HIBA-fed 1 day adults. Histograms show the transcript levels of genes involved in lipid metabolism in pep-2 mutants, supplemented with 10 mM 2-HIBA, as compared to untreated control. Experiments were performed in triplicate. Data are presented as mean ± SD (***p < 0.001, ns: not significant).