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RT-qPCR analysis of lipid metabolism genes in HGD worms. (A) Expression of sams-1, sbp-1, fat-7, acs-2, fasn-1, ech-1 and pmt-1 genes in 2-HIBA HGD 1-day adults, compared to HGD untreated nematodes. (B) Expression of lipid metabolism genes in untreated populations, grown with (HGD) or without (No-GD) glucose. Experiments were performed in triplicate. Data are presented as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001, ns: not significant).
