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. 2022 Nov 23;9:986022. doi: 10.3389/fmolb.2022.986022

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Copyright © 2022 Schifano, Conta, Preziosi, Ferrante, Batignani, Mancini, Tomassini, Sciubba, Scopigno, Uccelletti and Miccheli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of 2-HIBA on oxidative stress responses. (A) Expression of daf-2, daf-16, sek-1, sod-3 and gst-4 genes in N2 worms treated with 5, 10 or 20 mM 2-HIBA and in untreated nematodes at day 1 of adulthood. Histograms show the expression of genes involved in oxidative stress detected by real-time PCR. (B) Measurement of ROS levels in N2 supplemented with 2-HIBA compared to untreated control. Experiments were performed in triplicate. Data are presented as mean ± SD (*p < 0.05, **p < 0.01 and ***p < 0.001; ns: not significant).