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. 2022 Nov 23;9:986022. doi: 10.3389/fmolb.2022.986022

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Copyright © 2022 Schifano, Conta, Preziosi, Ferrante, Batignani, Mancini, Tomassini, Sciubba, Scopigno, Uccelletti and Miccheli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescence analysis of daf-16::GFP transgenic strain. (A) Effect of 10 mM 2-HIBA treatment on localization of DAF-16 protein and (B) respective Mean Fluorescence Intensity evaluation. Data were obtained from three independent experiments (60 worms for each condition). Scale bar = 100 μm control: untreated nematodes. Statistical analysis was performed by one-way ANOVA with the Bonferroni post-test; asterisks indicate significant differences (***p < 0.001). Bars represent the mean of three independent experiments.