TABLE 1.
Lifespan analysis of wild-type N2, sek-1, pmk-1, skn-1 mutant worms supplemented with 2-HIBA in standard or HGD condition. The lifespan of untreated animals was reported as control. Three experiments were performed in triplicate for each condition.
| C. elegans strain | Diet | n | Median lifespan | Maximum lifespan | Number of censored | Statistics |
|---|---|---|---|---|---|---|
| Wild-type N2 | Heat killed E. coli OP50 | 180 | 12 ± 0.2 | 20 ± 0.3 | 10 | - |
| Wild-type N2 | Heat killed E. coli OP50+ 0.1 mM 2-HIBA | 180 | 13 ± 1.2 | 22 ± 1.1 | 12 | ns versus N2 untreated control |
| Wild-type N2 | Heat killed E. coli OP50 + 5 mM 2-HIBA | 180 | 15 ± 0.5 | 34 ± 0.2 | 14 | p < 0.05 versus untreated N2 control |
| Wild-type N2 | Heat killed E. coli OP50 + 10 mM 2-HIBA | 180 | 26 ± 0.08 | 35 ± 0.3 | 13 | p < 0.01 versus untreated N2 control |
| Wild-type N2 | Heat killed E. coli OP50 + 20 mM 2-HIBA | 180 | 20 ± 1.4 | 30 ± 0.5 | 18 | p < 0.05 versus N2 untreated control |
| Wild-type N2 | Heat killed E. coli OP50 + 50 mM2-HIBA | 180 | 10 ± 1.3 | 17 ± 1.3 | 12 | p < 0.001 versus untreated N2 control |
| Wild-type N2 | Heat killed E. coli OP50 + 100 mM 2-HIBA | 180 | 8 ± 0.9 | 14 ± 0.09 | 14 | p < 0.001 versus untreated N2 control |
| sek-1 mutant | Heat killed E. coli OP50 | 180 | 13 ± 0.05 | 17 ± 0.5 | 10 | - |
| sek-1 mutant | Heat killed E. coli OP50 + 10 mM 2-HIBA | 180 | 12 ± 1.3 | 16 ± 0.1 | 15 | ns versus sek-1 untreated control |
| pmk-1 mutant | Heat killed E. coli OP50 | 180 | 13 ± 0.03 | 17 ± 0.9 | 15 | - |
| pmk-1 mutant | Heat killed E. coli OP50 + 10 mM 2-HIBA | 180 | 12 ± 0.06 | 17 ± 0.05 | 17 | ns versus pmk-1 untreated control |
| skn-1 mutant | Heat killed E. coli OP50 | 180 | 11 ± 0.1 | 15 ± 1.3 | 18 | - |
| skn-1 mutant | Heat killed E. coli OP50 + 10 mM 2-HIBA | 180 | 11 ± 1.2 | 15 ± 0.7 | 12 | ns versus skn-1 untreated control |
| pep-2 mutant | Heat killed E. coli OP50 | 180 | 13 ± 0.3 | 25 ± 0.08 | 19 | - |
| pep-2 mutant | Heat killed E. coli OP50 + 10 mM 2-HIBA | 180 | 13 ± 0.9 | 25 ± 0.4 | 17 | ns versus pep-2 untreated control |
| Wild-type N2 | HGD- Heat killed E. coli OP50 | 180 | 5 ± 0.8 | 11 ± 1.2 | 20 | - |
| Wild-type N2 | HGD-Heat killed E. coli OP50 + 5 mM 2-HIBA | 180 | 10 ± 1.2 | 16 ± 0.8 | 18 | p < 0.01 versus N2 untreated control |
| Wild-type N2 | HGD- Heat killed E. coli OP50 + 10 mM 2-HIBA | 180 | 7 ± 0.9 | 17 ± 0.4 | 15 | p < 0.01 versus N2 untreated control |
| Wild-type N2 | HGD- Heat killed E. coli OP50 + 20 mM 2-HIBA | 180 | 7 ± 1.4 | 15 ± 1.3 | 19 | p < 0.05 versus N2 untreated control |