The ΔTma values (°C) of studied ds-polynucleotides upon addition of ratio rb = 0.3 and of 15a–c, 16a, 16c, 17a, 21a and 21b at pH 7.0 (sodium cacodylate buffer, I = 0.05 mol dm−3).
| r b = 0.3 | 15a | 15b | 15c | 16a | 16c | 17a | 21a | 21b |
|---|---|---|---|---|---|---|---|---|
| ctDNA | 3.3 | 2.4 | 1.6 | 4.4 | 2.6 | 3.8 | 0 | 0 |
| Poly A–poly U | 3.7 | 1.0 | 0 | 0 | 0 | 0 | NDc | NDc |
| Poly(dA–dT)2 | 16.9 | 16.0 | 16.9 | 11.0 | 12.1 | 11.9 | 3.1 | 3.8 |
Difference between Tm value of free polynucleotide and complex with a small molecule; error in ΔTm: ±0.5 °C.
r = [compound]/[polynucleotide].
Not determined.