All sequences used are displayed. The biophysical screens are DSF, STD, ESI-MS and ¹H,¹⁵N-HSQC. For crystallization a construct lacking the linker GGS was used.

Construct	Sequence (N′–C′)
T-p53C-Y220C (biophysical screens)	GGSSS SVPSQ KTYQG SYGFR LGFLH SGTAK
	SVTCT YSPAL NKLFC QLAKT CPVQL WVDST
	PPPGT RVRAM AIYKQ SQHMT EVVRR CPHHE
	RCSDS DGLAP PQHLI RVEGN LRAEY LDDRN
	TFRHS VVVPC EPPEV GSDCT TIHYN YMCYS
	SCMGG MNRRP ILTII TLEDS SGNLL GRDSF
	EVRVC ACPGR DRRTE EENLR KKGEP HHELP
	PGSTK RALPN NT
T-p53C-Y220C (crystallization)	SSSVP SQKTY QGSYG FRLGF LHSGT AKSVT
	CTYSP ALNKL FCQLA KTCPV QLWVD STPPP
	GTRVR AMAIY KQSQH MTEVV RRCPH HERCS
	DSDGL APPQH LIRVE GNLRA EYLDD RNTFR
	HSVVV PCEPP EVGSD CTTIH YNYMC YSSCM
	GGMNR RPILT IITLE DSSGN LLGRD SFEVR
	VCACP GRDRR TEEEN LRKKG EPHHE LPPGS
	TKRAL PNNT
T-p53C-Y220C (C124/182/277S)	GGSSS SVPSQ KTYQG SYGFR LGFLH SGTAK
	SVTST YSPAL NKLFC QLAKT CPVQL WVDST
	PPPGT RVRAM AIYKQ SQHMT EVVRR CPHHE
	RSSDS DGLAP PQHLI RVEGN LRAEY LDDRN
	TFRHS VVVPC EPPEV GSDCT TIHYN YMCYS
	SCMGG MNRRP ILTII TLEDS SGNLL GRDSF
	EVRVC ASPGR DRRTE EENLR KKGEP HHELP
	PGSTK RALPN NT