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. 2022 Dec 14;17(12):e0279010. doi: 10.1371/journal.pone.0279010

Fig 5. Knockdown of COX2 ameliorated the ferroptosis sensitivity of cells cultured in high glucose.

Fig 5

(A) Immunofluorescence analysis with GPX4 antibody (red) and nuclear staining with DAPI (blue) in the HK-2 cells of the Si-Ctrl group, Si-COX2 group, HG+Si-Ctrl group and HG+Si-COX2 group. (B) Western blotting showed the protein expression of COX2, GPX4, SLC7A11, FTH-1 and TFR-1 in the cells of each group. G) GPX4 (C), SLC7A11 (D), FTH-1 (E), TFR-1 (F) and PTGS2 (G) mRNA expression in cells in each group. (H-J) GSH (H), MDA (I), 4-HNE(J)and iron concentrations (K) of cells in each group. (K-L) KIM-1 (L) and NGAL (M) increased mRNA expression was alleviated by transfection of COX2-siRNA in high glucose. Data are expressed as the mean ± SD. **p < 0.05 vs. Si-Ctrl group; ***p < 0.05 vs. HG+Si-Ctrl group.