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. 2022 Dec 14;17(12):e0279010. doi: 10.1371/journal.pone.0279010

Fig 6. Cellular ferroptosis induced by RSL-3 was alleviated by Fer-1 and aspirin.

Fig 6

(A)DCHF-DA staining revealed the generation of ROS (green) in the HK-2 cells cultured in 100 nmol/L RSL-3 medium compared to the control cells, and it was partially reduced by aspirin. (B) Cell viability was measured in HK-2 cells cultured with different concentrations of RSL-3 for 48 h, and it was partially improved by 400 nm Fer-1 or 400 μm aspirin treatment. (C) Cell viability measurements in HK-2 cells treated with RSL-3 100 nm for 48 h were partially improved by treatment with different concentrations of Fer-1 or AS. (D) Western blot analysis of SLC7A11, GPX4, FTH-1, TFR-1 and COX2 protein expression of cells in Ctrl, DMSO, RSL-3 100 nm, RSL-3 100 nm+Fer-1 400 nm, RSL-3 100 nm+AS400 μm and AS 400 μm groups. (E-I) GPX4 (E), SLC7A11 (F), FTH-1 (G), TFR-1 (H) and PTGS2 (I) mRNA expression in cells of each group. (J-M) GSH (J), MDA (K), 4-HNE(L)and iron concentrations (M) in the cells of each group. (N-O) The increased mRNA expression of KIM-1 (N) and NGAL (O) induced by RSL-3 was relieved by treatment with Fer-1 or aspirin. Data are expressed as the mean ± SD. **p < 0.05 vs. Ctrl group; ***p < 0.05 vs. RSL-3 group.