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. 2022 Dec 14;17(12):e0279010. doi: 10.1371/journal.pone.0279010

Fig 7. Cellular ferroptosis-induced RSL-3 was inhibited by transfection with COX2-siRNA.

Fig 7

(A) Immunofluorescence analysis with GPX4 antibody (red) and nuclear staining with DAPI (blue) of HK-2 cells in the Si-Ctrl, Si-COX2, RSL-3+Si-Ctrl, RSL-3+Si-COX2 and DMSO+Si-Ctrl groups. (B) The cell viability measurements in HK-2 cells treated with RSL-3 100 nm for 48 h were partially improved by transfection of COX2-siRNA. (C) Western blotting results of COX2, GPX4, SLC7A11, FTH-1 and TFR-1 in cells in each group. (D-H) The mRNA expression of GPX4 (D), SLC7A11 (E), FTH-1 (F), TFR-1 (G) and PTGS2 (H) in each group of cells. (I-L) GSH (I), MDA (J), 4-HNE(K) and iron concentrations (L) of HK-2 cells in the Si-Ctrl, Si-COX2, RSL-3+Si-Ctrl and RSL-3+Si-COX2 groups. (M-N) KIM-1 (M) and NGAL (N) increased mRNA expression induced by RSL-3 and were relieved by transfection of COX2-siRNA. Data are the mean ± SEM. **p < 0.05 vs. Si-Ctrl group; ***p < 0.05 vs. Si-Ctrl+ RSL-3 group.