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. 2020 Apr 7;43(1):158–161. doi: 10.1097/IAE.0000000000002800

Fig. 1.

Fig. 1.

A. Fluorescence (relative fluorescence units; RFU) versus concentration (ng/mL) curves for pure sodium fluorescein in solution. Excitation wavelength 481 nm; analysis/emission wavelength 522 nm. B. Fluorescence (relative fluorescence units; RFU) versus concentration (ng/mL) curves for pure sodium fluorescein and bloodstained sodium fluorescein in solution. Excitation wavelength 481 nm; analysis/emission wavelength 522 nm. The addition of blood results in a significant decrease in fluorescence (P = 0.004). C. Fluorescence (relative fluorescence units; RFU) versus concentration (ng/mL) curves for pure sodium fluorescein and bloodstained sodium fluorescein in solution following centrifuging (see text for details). Excitation wavelength 481 nm; analysis/emission wavelength 522 nm. Note the curves are near-identical.