Figure S6.

Relative position of the catalytic site in microtubule–VASH1 and –VASH2 complexes with respect to the C-terminus of helix H12 of α-tubulin. The centroid of the catalytic residue triad is displaced by 6.3 Å in VASH1 (Li et al., 2020) compared to VASH2 due to the observed tilt between the two enzyme complexes (Fig. 4). However, the distance between the catalytic triad centroid of VASH1 and VASH2 to the Cα atom of residue S439 of helix H12 of a-tubulin is very similar (29.7 versus 27.3 Å, respectively).