FIG. 5.

Immunoprecipitation of [³⁵S]methionine-labeled polypeptides from extracts of P. yoelii yoelii YM parasites with a panel of MAbs from hybridomas generated by immunization with purified PyAMA-1. Bound proteins were washed in a buffer containing 1% NP-40 and then washed in a buffer containing 0.1% SDS before elution in SDS-PAGE sample buffer. Bound proteins were separated by SDS-PAGE on a 10% polyacrylamide gel, and labeled proteins were detected by fluorography. The tracks contain immunoprecipitates with purified normal mouse IgG (track 1), rat MAb 28G2dc1 coupled to Q-Sepharose (track 2), mouse MAb 45B1 (track 3), mouse MAb 48F8 (track 4), and mouse MAb 48B2 (track 5). All mouse MAbs were coupled to protein G-Sepharose. The mobilities of molecular mass markers are indicated at the left; positions of the 140-kDa species and the 60-kDa AMA-1 are indicated by arrows on the right.