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. 2000 May;68(5):2899–2906. doi: 10.1128/iai.68.5.2899-2906.2000

FIG. 7.

FIG. 7

Single- and dual-antibody immunofluorescence analysis of the subcellular location of PyAMA-1 and the 140-kDa protein in fixed and unfixed free merozoites and in mature schizonts of P. yoelii yoelii YM. (A to C) Colocalized apical and surface immunofluorescence of PyAMA-1-specific MAb 45B1 (A) and AMA-1 C-terminal sequence-specific MAb 28G2dc1 (B) on fixed free merozoites and, for comparison, the reaction of MAb 28G2dc1 with mature schizonts is (C); (D and F) colocalized apical immunofluorescence of MAb 28G2dc1 and 140-kDa protein-specific MAb 48F8 on fixed free merozoites, respectively; (E) reaction of MAb 48B2 with mature schizonts and free merozoites; (G and H) surface reactivity of MAbs 45B1 and 48F8 with free and unfixed merozoites, respectively.