FIG. 8.
Immunoprecipitation of [35S]methionine-labeled polypeptides from P. falciparum FCB-1 parasite extracts with MAbs. Bound proteins were washed in a buffer containing 1% NP-40 and eluted in SDS-PAGE sample buffer. Proteins were separated on by SDS-PAGE on a 7.5% polyacrylamide gel, and labeled proteins were detected by fluorography. Tracks: 1, MAb 58F8dc1; 2, 28G2dc1; 3, 45B1; 4, 48F8; 5, 48B2. Both rat MAbs were coupled to Q-Sepharose, and all mouse MAbs were coupled to protein G-Sepharose. The mobilities of molecular mass markers are noted at the left; arrows indicate positions of the 83-kDa PfAMA-1 and the processing product.