FIG. 10.

Localization of hBD-2 peptide in F. nucleatum cell wall-stimulated cultured epithelial cells. HGE grown on coverslips were stimulated with 10 μg of F. nucleatum cell wall/ml for 24 h (Stim.) or left unstimulated (Unstim.) for the same time. The cells were fixed and reacted with polyclonal antibody against hBD-2, fluorescein isothiocyanate-conjugated secondary antibody (green), and, briefly, DAPI (blue) as described in Materials and Methods. Note the punctate localization of the signal, which is concentrated adjacent to the nucleus (arrow). The arrowhead indicates no immunoreactivity with polyclonal antibody against hBD-2 in another cell. Magnification, ×75. The data shown are representative of three independent experiments. F. nucleatum-stimulated HGE incubated with normal rabbit serum as a negative control showed no reactivity (data not shown).