FIG. 5.

Time-dependent production of MIP-2 mRNA in lung homogenates after instillation of LPS. Total RNA obtained from the lungs before and after LPS challenge was prepared at each time point (0, 1, and 3 h). Reverse transcription-PCR analysis was performed using MIP-2 and β-actin primers. The top band shows the PCR product of β-actin, and the bottom band shows the PCR product of MIP-2. The conditions of LPS administration are the same as in Fig. 1.