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. 2022 Dec 15;31(3):774–787. doi: 10.1016/j.ymthe.2022.12.008

Figure 4.

Figure 4

HK-2 cellular injury induced by ORF3A is mediated by upregulation of TRIM59 and STAT3 signaling activities

(A) Western blots for protein lysates from HK-2 cells. Expression of GFP, TRIM59, or ORF3A-v5 was achieved by Viafect transfection. TRIM59 was knocked down by shRNA. (B) Densitometric quantitation of western blot signals shown in (A). (C) Quantitative real-time PCR for HK-2 cells shown in (A). (D) Western blots for protein lysates from HK-2 cells. Expression of GFP, TRIM59, or dominant-negative STAT3 (DN-STAT3) was achieved by lentiviral transduction. (E) Densitometric quantitation of western blot signals shown in (D). (F) Quantitative real-time PCR for HK-2 cells shown in (D). ∗p < 0.05; ∗∗p < 0.01.