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. 2022 Dec 15;31(3):774–787. doi: 10.1016/j.ymthe.2022.12.008

Figure 6.

Figure 6

SARS-CoV-2 ORF3A enhances STAT3 signaling and exacerbates kidney injury in mice with ischemia-reperfusion

(A) Schematic graph showing the timeline of the experimental procedures. (B) Western blots for protein lysates from mouse kidneys following ORF3A expression (3A) and ischemia-reperfusion (I/R). (C) Densitometric quantitation of western blot results shown in (B) (normalized to Vaculin) and the mRNA level of KIM-1 measured by quantitative real-time PCR (normalized to 18S rRNA). (D) Measurements of BUN and serum creatinine in mice. (E) Quantitative PCR measurements of mRNA levels of STAT3 target genes, normalized to 18S rRNA. (F) Immunohistochemical staining of mouse kidney sections. (G) Quantification of IHC staining results shown in (F). ∗p < 0.05; ∗∗p < 0.01.