FIG. 7.

Survival of internalized bacteria in adherent and suspended D. discoideum. D. discoideum was incubated in MB medium either as adherent monolayers in tissue culture wells or as suspended cells shaken in tubes. Cells were infected with bacteria for 30 min, at which point gentamicin was added to kill extracellular bacteria. Viable, intracellular bacteria remaining were counted by measuring CFU extracted from washed cells. The experiment was performed twice, each condition was done in duplicate, and the error bars indicate the n − 1 weighted sample standard deviation.