FIG. 9.

Growth of L. pneumophila in D. discoideum mutants. Various D. discoideum mutants and their parent strains were plated in tissue culture wells in MB medium and infected with L. pneumophila Philadelphia-1 at an MOI of 1:1. The number of viable bacteria was determined by counting CFU. The experiments were performed two to four times depending on the strain, each point in the experiment was done in triplicate, and the error bars indicate the n − 1 weighted sample standard deviation.