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. 2022 Nov 30;13:1036196. doi: 10.3389/fimmu.2022.1036196

Figure 5.

Figure 5

Effect of B. vulgatus Bv46 on the expression of Ccl19, Cd19, Cd22, Cd40 and Cxcr5 genes in mice colon and the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS in vitro. CT, DSS, and BV denote control (no DSS induction), DSS induction and treatment with phosphate buffer saline only, and DSS induction and treatment with B. vulgatus Bv46 respectively. (A) Effects of B. vulgatus Bv46 administration on the expression of inflammatory genes in DSS-induced colitis mice. Mouse colons were collected and the mRNA expression of Ccl19, Cxcr5, Cd19, Cd22, Cd40 was quantified using real-time PCR. Fold changes are expressed as mean ± SD (n = 3 each group). *P < 0.05, **P < 0.01. (B) The cytokine profiles (concentrations of TNF-α, IL-1β and IL-6) of the supernatants after the cultivation of untreated RAW264.7 cells or cells after treatment with LPS or LPS+BV. Error bars are SD. *P < 0.05 for LPS+BV versus LPS. NEG, negative control.