Figure 1.

Experimental procedure. The baseline ABR of mice was measured 7 d before noise exposure. Mice were administered Fer-1 or vehicle (saline and 20% SBE-β-CD) by i.p. injection 24 h and 1 h before noise exposure. Then, mice were exposed to 99~100 dB SPL 2-20 kHz BBN for 2 h. The mice received the third injection immediately after the noise was turned off. Mice used for the analysis of oxidative stress levels were sacrificed 1 h after the end of noise exposure. Mice used for analysis of the effects of Fer-1 treatment on auditory threshold shifts were administered two more i.p. injections the next day (in the a.m. and p.m.). After ABR testing 14 d following noise exposure, the mice were sacrificed to evaluate OHC loss and IHC synapse loss. Twenty-eight days after noise exposure, the mice were sacrificed for evaluation of ANF degeneration. BBN: broadband noise.