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. 2022 Jun 20;40(12):1855–1861. doi: 10.1038/s41587-022-01364-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a) Y2H data of sensor hits; each row shows the response of an evolved PYR1 sensor to its target ligand; the sequences of the sensors used are shown in supporting data file 1. (b) Chemical structures of compounds for which at least one PYR1-based biosensor was identified. (c) Chemical structures of compounds for which no PYR1-based biosensor was identified.