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. 2022 Jun 14;29(12):1854–1865. doi: 10.1038/s41417-022-00484-z

Fig. 4. TLR9 agonist-treated huPBMC reduced MDSC population and increases IFNα- and NFκB-regulated genes.

Fig. 4

A Gating strategy for phenotypic analysis of MDSCs; isolated human PBMCs were treated with increasing concentrations (0.04–10 µM) of SD101, ODN2395 along with ctrl ODN5328 (1 µM) for 48 h. B MDSC population was quantified followed by FC analysis. Four donors with three replicates were used, and data represented as mean ± SEM (n = 12). C Supernatants of Donor 1 and 2 were collected analyzed for (i) IL29, (ii) IFNα, (iii) IL6, (iv) IL10 by using Luminex. Cells treated with SD101 and ODN2395 are represented as red and blue boxes, respectively. Data from two representative donors with two replicates are presented here. For Donor 1 and 2 supernatants from 10 µM ODN2395 treated samples were unavailable for luminex potential analysis.