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. 2022 Jun 14;29(12):1854–1865. doi: 10.1038/s41417-022-00484-z

Fig. 7. Locoregional TLR9 stimulation with systemic CPI effectively inhibits tumor progression.

Fig. 7

A Schema illustrates the steps to develop LM and the treatment regimen. Eight- to twelve-week-old male C57/BL6 mice were challenged using intra-splenic injection model with MC38-CEA-Luc cells for 1 week. Bioluminescence was measured by IVIS and mice were randomized on D0 prior to treatment with 30 µg/mouse ODN2395 via PV with or without 250 µg/mouse of anti-PD1 antibody via IP on D0, D3, and D6. PBS-treated mice via PV were used as control. B Tumor growth was monitored by IVIS imaging on D2, D4, D7, D10, and D12. Fold change of the tumor burden was calculated based on D0 baseline bioluminescence. Data was analyzed by multiple t test. Discovery determined using the two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 1%.